Research Note— Serotyping of Avibacterium paragallinarum Isolates from Peru
نویسندگان
چکیده
Laboratorio de Biologı́a Molecular, Facultad de Farmacia y Bioquı́mica – Universidad Nacional Mayor de San Marcos, Lima 1, Perú Instituto Nacional de Tecnologı́a Agropecuaria (INTA), Estación Experimental Agropecuaria (EEA) Balcarce, Departamento de Producción Animal, CC 276, 7620, Balcarce, Buenos Aires, Argentina Laboratorio de Investigación y Desarrollo, División de Producción Animal – Innova Andina S.A., Lima 27, Perú Bioservice S.R.L., Lima 35, Perú
منابع مشابه
16S ribosomal RNA sequencing and molecular serotyping of Avibacterium paragallinarum isolated from Indian field conditions
AIM This study was aimed at identifying Indian field isolates of Avibacterium paragallinarum on both molecular as well as serological levels that cause infectious coryza in chickens. MATERIALS AND METHODS Species-specific polymerase chain reaction (HPG-2 PCR), and 16S ribosomal RNA (rRNA) sequencing were employed for molecular identification. Whereas, multiplex PCR technique was used for sero...
متن کاملRetrospective Detection of Avibacterium Paragallinarum Serovar B in Egg Yolk Materials by PCR
Avibacterium paragallinarum is Gram-negative bacteria that cause infectious coryza (IC), an acute respiratory disease of chickens. Despite vaccination of Layer and breeder farms of Iran against IC, they are still experiencing the disease clinically and there is no knowledge of serotypes prevalence of this bacterium in this country. This study designed to determine serovar identity by molecular ...
متن کاملIsolation, Identification and Antibiogram Pattern of Avibacterium Paragallinarum from Japanese Quails
A total of 53 trachea, air sacs, lung, and infra orbital sinus exudates were collected from 5 commercially reared Japanese quail farms showing typical symptoms of infectious coryza. Avibacterium paragallinarum was isolated and identified using selective media and by biochemical identification methods. Biochemical identification tests such as satellitism, IMViC tests and carbohydrate fermentatio...
متن کاملAmplified 16S ribosomal DNA restriction analysis for identification of Avibacterium paragallinarum.
A molecular technique based on the restriction fragment length polymorphism of the 16S ribosomal genes amplified by a polymerase chain reaction (PCR), referred to as amplified 16S ribosomal DNA restriction analysis (ARDRA), was designed to identify 19 Avibacterium paragallinarum strains isolated from infraorbital sinus and nasal turbinate bone samples of broiler chickens, breeders, and laying h...
متن کاملReclassification of Pasteurella gallinarum, [Haemophilus] paragallinarum, Pasteurella avium and Pasteurella volantium as Avibacterium gallinarum gen. nov., comb. nov., Avibacterium paragallinarum comb. nov., Avibacterium avium comb. nov. and Avibacterium volantium comb. nov.
This paper describes a phenotypic and genotypic investigation of the taxonomy of [Haemophilus] paragallinarum, Pasteurella gallinarum, Pasteurella avium and Pasteurella volantium, a major subcluster within the avian 16S rRNA cluster 18 of the family Pasteurellaceae. An extended phenotypic characterization was performed of the type strain of [Haemophilus] paragallinarum, which is NAD-dependent, ...
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تاریخ انتشار 2009